Screening and evaluation of saline-alkali-tolerant and growth-promoting bacteria / 生物工程学报

Salinity is the most important factor for the growth of crops. It is an effective method to alleviate the toxic effect caused by salt stress using saline-alkali-tolerant and growth-promoting bacteria in agriculture. Seven salt-tolerant bacteria were screened from saline-alkali soil, and the abilities of EPS production, alkalinity reduction and IAA production of the selected strains were investigated. A dominant strain DB01 was evaluated. The abilities of EPS production, alkalinity reduction and IAA production of strain DB01 were 0.21 g/g, 8.7% and 8.97 mg/L, respectively. The isolate was identified as Halomonas aquamarina by partial sequencing analysis of its 16S rRNA genes, and had the ability to inhibit the growth of Fusarium oxysporum f. sp., Alternaria solani, Phytophthora sojae and Rhizoctonia cerealis. It also could promote root length and germination rate of wheat seedlings under salt stress. Halomonas aquamarina can provide theoretical basis for the development of soil microbial resources and the application in saline-alkali soil improvement.

Determination of Six Index Components in Xiaoyao Pills by Quantitative Analysis of Multi-components by Single Marker / 中国药师

Objective:To develop a method of quantitative analysis of multi-components by single marker (QAMS) for six active constituents in Xiaoyao pills. Methods:Using glycyrrhizic acid as the reference, an HPLC method with a Kromasil C18 column (250 mm ×4.6 mm,5 μm) was applied,the mobile phase was acetonitrile(A)-0.1% phosphoric acid solution(B) with gradient elution (0-10 min,5 % A;10-25 min,5 % →20 % A;20-70 min,15 % →60 % A and 40-60 min,60 % A). The detection wavelength was 280nm,the column temperature was 35℃ and the injection volume was 10 μl. The relative correction factors among the six index components were detected by QAMS. The contents of the six index components were determined by an external standard method and QAMS to compare the results obtained from the two different methods and verify the practicability and stability of QAMS. Results:The established QAMS was used to determine the six index components in Xiaoyao pills,and totally 6 batches of Xiaoyao pills were determined.There were no significant differences in the calculated values and the determined ones (P > 0.05). Conclusion:The QAMS method is simple,effective and accurate in determining the contents of the six index components in Xiaoyao pills,which can be used for the quality control of Xiaoyao pills and provide reference for the further study.

Portal CO_2-DSA with fine needle splenic puncture in an animal model / 介入放射学杂志

Objective To evaluate the safety and feasibility of portal CO 2-DSA with fine needle splenic puncture. Methods The splenic tails of seven adult white rabbits were exteriorized by laparotomy, and followed by a 25 gauge fine needle inserting about 1.0 cm into the splenic parenchyma. Portal CO 2-DSA was performed (2.0 ml/s, 10ml) and the images were evaluated. After removal of the needle, the puncture site was observed for bleeding till coagulation occurred. The spleen were taken for gross and histological examination. Results All the CO 2-DSA clearly showed the portal trunk with intrahepatic branches above 3～4 orders, the main splenic vein, and the main mesenteric veins with parts of its branches. CO 2 disappeared from the intrahepatic portal vein over 2～3 minutes. In one animal, the left renal vein and the inferior vena cava were also displayed by CO 2 through communication between splenic vein and renal vein. After removal of the needle, there was small amount of bleeding at the puncture site which ceased spontaneously over 3～5 minutes. In all animals, no extravasation of CO 2 at the puncture site, no subcapsular dissection or intrasplenic hematoma was observed. Microscopically, the splenic capsule appeared intact and there was no evidence of subcapsular hematoma formation.Conclusions Portal CO 2-DSA with fine needle splenic puncture is feasible, safe and efficient. In normal adult rabbit, CO 2 may help to visualize the left renal vein and inferior vena cava through communication between splenic and renal vein.

Venous stenosis model induced by local mechanical injury with the presence of surgical arteriovenous fistula in swine / 介入放射学杂志

Objective To evaluate a new animal model of venous stenosis induced by local mechanical injury with the presence of surgical arteriovenous fistula (AVF).Methods Twelve arteriovenous (AV) fistulae were surgically formed between the carotid artery and internal jugular vein in six adult pigs, one on each side of the neck. Direct mechanical injury was made by crush injury with fingers or forceps to the jugular vein at the sites 1-2cm above and below the AV anastomosis. Angiographic follow up was performed at 3 and 6 weeks, and the animals were sacrificed. Fistulae and injured veins were harvested for histopathology. Results At angiography six AV anastomoses were patent without stenosis, five were stenosed and one had occluded. Eleven of twelve venous injury sites with open AV anastomosis and six of ten venous injury sites with AV anastomotic stenoses developed greater than 50% diameter stenosis. Dilation was found in the non injury segment of eight jugular veins. Stenoses were caused by neointimal hyperplasia as seen on histologic examination.Conclusions Neointimal venous stenosis can be induced by creation of a surgical AV fistula and local venous mechanical injury. This model may be used to study methods to reduce or inhibit neointimal hyperplasia, with particular reference to venous stenoses that occur in arteriovenous shunts created for dialysis access.

The Effect of NK Cell Function in Peripheral Blood of Patients with Advanced Primary Liver Cancer During Interventional Treatment / 介入放射学杂志

NK cell function was tested in 60 patients randomly selected with ad- vanced primary liver cancer(PLC)during interventional treatment.The patients were di- vided into three groups and treated with different regimens.In group 1(n=20),routinally using HAI(5-Fu 1000mg,CDDP 30mg)and HAE(Lipiodel 10～20ml,EAMC 50mg)in group 2,the treatment project is same as in group 1,but after the treatment,total 8g Natrii thiosulfas was administrated intravenously within 48 hours;in group 3,one week after treatment as in group 1,the patients received a-2b IFN injected subcutaneously at a dose of 10?10~6 IU,B.i.w,for more than 2 months.The results showed that:1.NK function in the patients with advanced PLC was 17.45?4.5% ,significantly lower than that in the nor- mal ones(21.39?3.58%);2.After the interventional treatment,the NK function of the patients were obviously decreased in group 1(13.58?3.14%),and in group 2(15.45?2. 69%),but increased in group 3(19.20?3.27%).The results suggested that conventional interventional treatment will affect NK cell function of patients with advanced PLC in some degree,and the effect can be reduced by injecting Natrii Thiosulfas i.v.,and administering IFN.